Journal: bioRxiv
Article Title: Extracellular vesicle bioactivity and potential clinical utility is determined by mesenchymal stromal cell clonal subtype
doi: 10.1101/2024.09.05.609844
Figure Lengend Snippet: In vitro treatment of activated CD4+ T cells with Y201 and Y202 EVs (n=2, mean events >18,000 counted) A) Proliferative cycles. B) Proliferative index C) Polarisation of activated T cells in the absence and presence of Y201 EVs and Y202 EVs (n=2, mean events >32,000 counted). D/E) Total peritoneal exudate cell (PEC) counts following zymosan (D) or schistosome egg (E) induced inflammation in the absence and presence of Y201 EVs and Y202 EVs. F/G) Examination of TCR+CD4+, naïve and central memory T cells in zymosan or schistosome egg induced inflammation in the absence and presence of Y201 EVs and Y202 EVs (n=3). One-Way ANOVA with Bonferroni post hoc testing,*p<0.05, **p<0.01, ***p<0.001.
Article Snippet: To determine MSC-derived EV immunomodulation for deactivation and suppression of T cell proliferation, suspension cultures of 1.0×10 5 primary human peripheral blood-derived CD4+ T cells (Stem Cell Technologies) were grown in RPMI-1640 with 10% FBS 1% P/S (Gibco, Cat: 10363083) and were pre-treated for 6hrs with 20X EVs isolated from serum-free conditioned medium collected over 24hrs culture from 2.0×10 6 of Y201 or Y202 MSCs.
Techniques: In Vitro